RBS

Part:BBa_K4632023

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


BBa_B0033 gain from literature

it is from the Registry of standard Biological Parts:BBa_B0033. [1] We gain this sequence from literature(Jiang et al.,2020) and use it in our Quorum sensing-based T4 lysis device.

bba-k4632023.png

ACS SYNTH BIOL, 2020

In order to regulate our lysis time and thereby control product concentration in our quorum-sensing-based T4 lysis device, we used this element in front of our T4 endolysin and regulated it.

See more detail in BBa_K4632024[2]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Jiang W, He X, Luo Y et al. Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control[J]. ACS SYNTH BIOL, 2020,9(9):2588-2599.


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